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Uploading files: FTP & Aspera

Once your submission files have been prepared using the EgaCryptor, the resulting encrypted files and associated md5sum files can be uploaded to your submission account using Aspera or FTP.

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Using Aspera to upload your prepared files

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Downloading the Aspera ascp command line program

Aspera is a commercial file transfer protocol that may provide faster transfer speeds than ftp especially over longer distances.

Operating System: Windows XP / 2003 / Vista / 2008 / 7 / 8, Mac OS Intel 10.5 / 10.6 / 10.7 / 10.8 / Linux

The Aspera ascp command line client can be downloaded here.

Please select *Aspera Connect*.

The ascp command line client is distributed as part of the aspera connect high-performance transfer browser plug-in and is free to use, without registration.

The location of the 'ascp' program in the filesystem:

Mac: on the desktop go cd /Applications/Aspera Connect.app/Contents/Resources/ there you'll see the command line utilities where you're going to use 'ascp'.

Windows: the downloaded files are a bit hidden. For instance in Windows 7 the ascp.exe is located in the users home directory in: AppDataLocalProgramsAsperaAspera Connectbinascp.exe

Linux: should be in your user's home directory, cd /home/username/.aspera/connect/bin/ there you'll see the command line utilities where you're going to use 'ascp'.

Using the Aspera ascp command line program

Your command should look similar to this:

or if you wish to upload several files at once without being asked for a password, please use the following command

Explanation of parameters

'-l300M' option sets the upload speed limit to 30MB/s. You may wish to lower this value to increase the
reliability of the transfer.

'-L-' option is for printing logs out while transferring

<files to upload> can be a file mask (e.g. '/homes/submitter/*.srf) or a list of files.

<ega-box-N> is your submission account log-in.

Add '-k2' switch for transfer restarts

Check the command line transfer usage for more configuration details.

Using FTP to upload your prepared files

i) Using default ftp command line client in Windows

1- Start the command line interpreter: press Win-R, type cmd, hit enter

2- Enter 'ftp ftp.ega.ebi.ac.uk'

3- Enter your submission username

4- Enter your submission password

5- Type 'binary' to enter binary mode for transfer

6- To see a list of available ftp commands type 'help'.

7- Type 'ls' command to check the content of your submission account.

8- Type 'prompt' to switch off confirmation for each file uploaded.

9- Use 'mput' command to upload files: 'mput *.bam*'

10- Use 'bye' command to exit the ftp client.

11- Use 'exit' command to exit the command line interpreter.

ii) Using default ftp command line client in Linux/Unix

1- Open a terminal and type 'ftp ftp.ega.ebi.ac.uk'

2- Enter your submission username

3- Enter your submission password

4- Type 'binary' to enter binary mode for transfer

5- To see a list of available ftp commands type 'help'.

6- Type 'ls' command to check the content of your drop box.

7- Type 'prompt' to switch off confirmation for each file uploaded.

8- Use 'mput' command to upload files: 'mput *.bam*'

9- Use 'bye' command to exit the ftp client.

iii) Using an FTP client

e.g. Filezilla

Use the following connection details (File=> Site Manager) and add your submission account username and password :

Select the files you wish to upload, then right click mouse, and select 'upload':

Submit to EGA

• Submission Tools

IBM® Aspera® software supports upgrades against the two most recent versions of a release. If you submit a ticket for a product that precedes the two most recent versions, you’ll be referred to IBM professional services for upgrade assistance.

• IBM Aspera Enterprise

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• IBM Aspera Sync

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• IBM Aspera Connect

• IBM Aspera Command Line Interface

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